The effect of 17β Estradiol Exposure on Mutant p53 Expression in Hydatidiform Mole Trophoblast Cell Culture

Abstract

Objectives: To compare the mutant p53 expression in normal trophoblast (N) cell culture with hydatidiform mole trophoblast (HM) cell culture which was exposed to 17β estradiol. Methods: An experimental study conducted at the Laboratory of Physiology Faculty of Medicine, Brawijaya University Malang using N cell culture and HM cell culture with 17β estradiol exposure. Trophoblast cell culture of normal and hydatidiform mole was divided in 6 groups, such as: 1. Without added 17β estradiol; 2. Added 5 nm 17β estradiol; 3. Added 10 nm 17β estradiol; 4. Added 20 nm 17β estradiol; 5. Added 40 nm 17β estradiol; 6. Added 80 nm 17β estradiol. Then performed immunocytochemistry staining using p53 mutant primary antibody and observed the expression of p53 mutant. Data from observations analized with the ANOVA test and correlation test. Results: Mutant p53 expression in N cell culture showed no significant differences in each treatment dose of 17β estradiol (p = 0086 > 0.05). The dose at 80nm 17β estradiol showed an average of highest mutant p53 expression on N cell culture rather than giving the dose of 17β estradiol on 40 nm, 20 nm, 10nm and 5 nm. While the control group showed a lowest average of mutant p53 expression in N cell culture when compared to the treatment group which was exposed to 17β estradiol. Mutant p53 expression in HM cell culture showed a significant difference at each treatment dose of 17β estradiol (p = 0.000 < 0.05). The existence of the effect of 17β estradiol begins when the expression of mutant p53 in HM cell culture becomes higher after being given treatment in the form of 17β estradiol on the dose of 5 nm compared with the expression of 17β estradiol in the control group. Then the expression of mutant p53 in HM cell culture is increasing when given doses of 17β estradiol at 20 nm and 40 nm. At a dose of 40 nm it shows the highest expression of mutant p53. Expression of mutant p53 in HM cell culture decreased when given at doses 80 nm. Conclusion: Mutant p53 expression in N cell culture exposed to 17β estradiol showed no significant difference. Expression of mutant p53 in HM cell culture which was exposed to 17β estradiol showed a significant difference. Mutant p53 expression in N and HM cell culture which was exposed to 17β estradiol showed significantly different, in which mutant p53 expression in N cell culture is lower than the expression of mutant p53 in HM tissue culture. [Indones J Obstet Gynecol 2011; 35-1: 30-5] Keyword: p53 mutant, 17β-estradiol, hydatidiform mole